CLASS-12-CHEMISTRY-LAB-MANNUAL-2026
Class XII Chemistry Practical Examination
Evaluation Scheme (Total Marks: 30)
| Component | Marks |
|---|---|
| Volumetric Analysis | 08 |
| Salt Analysis | 08 |
| Content Based Experiment | 06 |
| Project Work | 04 |
| Class Record & Viva Voce | 04 |
| Total | 30 |
Practical Syllabus
Micro-chemical methods are available for several practical experiments. Wherever possible, such techniques should be used.
A. Surface Chemistry
- Preparation of one lyophilic and one lyophobic sol
- Dialysis of prepared sol
- Role of emulsifying agents in stabilizing emulsions
Readings: Observation of sol formation, stability, color and dispersion medium.
Result: Lyophilic sols were found to be more stable than lyophobic sols.
Conclusion: Emulsifying agents increase stability by reducing surface tension.
B. Chemical Kinetics
- Effect of concentration and temperature on rate of reaction between Na2S2O3 and HCl
- Iodide–Hydrogen Peroxide reaction OR Clock reaction
Readings: Time taken for turbidity or color change at different concentrations.
Result: Rate of reaction increases with increase in concentration and temperature.
Conclusion: Reaction rate is directly proportional to concentration and temperature.
C. Thermochemistry
- Enthalpy of dissolution
- Enthalpy of neutralization
- Hydrogen bond formation experiment
Readings: Temperature change before and after reaction.
Result: Neutralization reactions were exothermic.
Conclusion: Heat change confirms energy transfer during chemical reactions.
D. Electrochemistry
Variation of cell potential of Zn/Zn2+ || Cu2+/Cu with concentration.
Readings: EMF values recorded at different electrolyte concentrations.
Result: EMF decreased with decrease in ion concentration.
Conclusion: Cell potential depends on electrolyte concentration.
E. Chromatography
- Separation of pigments
- Determination of Rf values
Readings: Distance traveled by solute and solvent front.
Result: Different pigments showed different Rf values.
Conclusion: Chromatography effectively separates components based on affinity.
F. Preparation of Inorganic Compounds
- Ferrous Ammonium Sulphate / Potash Alum
- Potassium Ferric Oxalate
Result: Crystalline inorganic compounds were successfully prepared.
Conclusion: Controlled crystallization improves purity.
G. Preparation of Organic Compounds
- Acetanilide
- Dibenzal Acetone
- p-Nitroacetanilide
- Aniline Yellow Dye
Readings: Melting point determination of prepared compound.
Result: Melting point matched standard values.
Conclusion: Purity of organic compound confirmed.
H. Functional Group Tests
Tests for unsaturation, alcohol, phenol, aldehyde, ketone, carboxylic acid and amine.
I. Biomolecules Tests
Detection of carbohydrates, fats and proteins in food samples.
J. Volumetric Analysis
Determination of molarity of KMnO4 using Oxalic Acid or FAS.
Result: Concentration of KMnO4 solution was determined accurately.
K. Qualitative Analysis
Identification of one cation and one anion from the given salt.
Final Conclusion: Systematic qualitative analysis helps in accurate identification of ions.
Detailed Results of Chemistry Practical Experiments
A. Surface Chemistry
1. Preparation of Lyophilic and Lyophobic Sol
Result:
The lyophilic sol (starch/egg albumin/gum) formed a stable colloidal solution and did not coagulate on standing.
The lyophobic sol (Fe(OH)₃/Al(OH)₃/As₂S₃) was less stable and showed coagulation on addition of electrolytes.
2. Dialysis of Sol
Result:
Impurities and excess electrolytes were successfully removed from the sol through the parchment membrane, increasing the stability of the colloidal system.
3. Emulsion and Emulsifying Agents
Result:
The emulsion prepared with emulsifying agents like soap or gum was more stable than the emulsion without emulsifiers.
B. Chemical Kinetics
1. Effect of Concentration and Temperature
Result:
The rate of reaction between sodium thiosulphate and hydrochloric acid increased with increase in concentration and temperature, as indicated by faster appearance of turbidity.
2. Iodide–Hydrogen Peroxide / Clock Reaction
Result:
Increase in iodide ion concentration reduced the time for color change, confirming that reaction rate depends on reactant concentration.
C. Thermochemistry
1. Enthalpy of Dissolution
Result:
Dissolution of potassium nitrate absorbed heat (endothermic), while copper sulphate released heat (exothermic), as indicated by temperature change.
2. Enthalpy of Neutralization
Result:
Neutralization of HCl with NaOH produced heat, confirming the reaction to be exothermic.
3. Hydrogen Bond Formation
Result:
Heat was evolved during interaction between acetone and chloroform, indicating hydrogen bond formation.
D. Electrochemistry
Result:
The EMF of Zn/Zn²⁺ || Cu²⁺/Cu cell decreased with decrease in electrolyte concentration, confirming dependence of cell potential on ion concentration.
E. Chromatography
1. Separation of Pigments
Result:
Different pigments separated at different heights on chromatogram and showed distinct Rf values.
2. Inorganic Ion Separation
Result:
Cations present in the mixture were successfully separated due to significant difference in their Rf values.
F. Preparation of Inorganic Compounds
Result:
Pure crystals of ferrous ammonium sulphate / potash alum / potassium ferric oxalate were obtained with proper shape and color.
G. Preparation of Organic Compounds
Result:
The prepared organic compound was obtained in good yield.
Its melting point was found close to the standard value, indicating high purity.
H. Functional Group Analysis
Result:
The given organic compound showed positive tests for the identified functional group based on characteristic color change or precipitate formation.
I. Tests for Carbohydrates, Fats and Proteins
Result:
Carbohydrates, proteins, and fats were successfully identified using Molisch’s, Biuret and Sudan tests, respectively.
J. Volumetric Analysis
Result:
The molarity of the KMnO₄ solution was determined accurately by titration against standard oxalic acid/ferrous ammonium sulfate solution.
K. Qualitative Analysis
Result:
The given salt contained one cation and one anion which were identified correctly using systematic qualitative analysis.
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